“Can sitting cause testicle pain?”

Testicular pain is not generally associated with sitting and can be a medical emergency if you have something like testicular torsion. That's the worst-case scenario, but scrotal hernias, testicle infections, or even sitting on the wrong bicycle seat could also be contributing to your pain. There are even other less emergent considerations, but bottom line is that you need a physical evaluation. While it may only hurt while you sit now, that pain may be your only clue to a more serious condition that is brewing.

Yes